Electrophoresis in Practice: A Guide to Methods and Applications of DNA and Protein Separations, 5th Edition
- Length: 458 pages
- Edition: 5
- Language: English
- Publisher: Wiley-VCH
- Publication Date: 2016-05-16
- ISBN-10: 3527338802
- ISBN-13: 9783527338801
- Sales Rank: #5843856 (See Top 100 Books)
This fifth edition of the successful, long-selling classic has been completely revised and expanded, omitting some topics on obsolete DNA electrophoresis, but now with a completely new section on electrophoretic micro-methods and on-the-chip electrophoresis.
The text is geared towards advanced students and professionals and contains extended background sections, protocols and a trouble-shooting section. It is now also backed by a supplementary website providing all the figures for teaching purposes, as well as a selection of animated figures tested in many workshops to explain the underlying principles of the different electrophoretic methods.
Table of Contents
Part I Fundamentals
Chapter 1 Electrophoresis
Chapter 2 Isotachophoresis
Chapter 3 Isoelectric Focusing
Chapter 4 High-Resolution Two-Dimensional Electrophoresis
Chapter 5 Protein Sample Preparation
Chapter 6 Protein Detection
Chapter 7 Blotting
Part II Equipment and Methods
Chapter 8 Special Laboratory Equipment
Chapter 9 Consumables
Chapter 10 Chemicals
Method 1 PAGE of Dyes
Method 2 Agarose and Immunoelectrophoresis
Method 3 Titration Curve Analysis
Method 4 Native PAGE in Amphoteric-Buffers
Method 5 Agarose IEF
Method 6 PAGIEF in Rehydrated Gels
Method 7 Horizontal SDS-PAGE
Method 8 Vertical PAGE
Method 9 Semidry Blotting of Proteins
Method 10 IEF in Immobilized pH Gradients
Method 11 High-Resolution 2D Electrophoresis
Method 12 PAGE of DNA Fragments
Donate to keep this site alive
1. Disable the AdBlock plugin. Otherwise, you may not get any links.
2. Solve the CAPTCHA.
3. Click download link.
4. Lead to download server to download.