Organ-on-a-Chip: Methods and Protocols
- Length: 334 pages
- Edition: 1
- Language: English
- Publisher: Springer
- Publication Date: 2021-09-15
- ISBN-10: 1071616927
- ISBN-13: 9781071616925
- Sales Rank: #0 (See Top 100 Books)
This book provides a collection of microphysiological systems employed for chemical/drug screening and strategies to mimic various physiological conditions. Chapters guide readers through Organ-on-a-Chip( OoC) platforms such as liver, intestine, blood-brain barrier, kidney, vessels, cardiac and skeletal muscles, articular joint, human fat. Additional chapters detail microfabrication technologies used to fabricate OoC devices such as, standard photo- and soft-lithography, techniques to fabricate membranes, and industrial-oriented fabrication methods.Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge, Organ-On-a Chip: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.
Cover Front Matter 1. Photo and Soft Lithography for Organ-on-Chip Applications 2. Organ-on-Chips for Studying Tissue Barriers: Standard Techniques and a Novel Method for Including Porous Membranes Within Microfluidic Devices 3. Thermoplastic Microfluidics 4. Bile Duct-on-a-Chip 5. Establishment of a Modular Anaerobic Human Intestine Chip 6. Intestinal Epithelium Tubules on a Chip 7. A Human Neurovascular Unit On-a-Chip 8. Generation of a Glomerular Filtration Barrier on a Glomerulus-on-a-Chip Platform 9. Electromechanical Stimulation of 3D Cardiac Microtissues in a Heart-on-Chip Model 10. Miniaturized Vascularized Bleeding Model of Hemostasis 11. Fabrication and Use of a Pumpless Microfluidic Lymphatic Vessel Chip 12. On-Chip Platelet Activation Assessment: Microfluidic Emulation of Shear Stress Profiles Induced by Mechanical Circulatory Support Devices 13. Biofabrication of 3D Human Muscle Model with Vascularization and Endomysium 14. Mechanical Induction of Osteoarthritis Traits in a Cartilage-on-a-Chip Model 15. Detailed Methodology to Establish a Synovium/Cartilage-on-a-Chip Model to Investigate the Process of Monocyte Extravasation 16. A Mesoscale 3D Culture System for Native and Engineered Biphasic Tissues: Application to the Osteochondral Unit 17. 3D Biofabricated In Vitro Models of Vascularized and Mineralized Bone Tissues 18. Isolation, Integration, and Culture of Human Mature Adipocytes Leveraging Organ-on-Chip Technology Back Matter
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